Gene Synthesis
Custom gene synthesis is the de novo chemical synthesis of any double-stranded DNA sequence without dependence on a biological template. Enzovera's gene synthesis service covers sequences from 50 bp to 200 kb, encompassing standard genes, complex regulatory elements, codon-optimized expression constructs, antibody variable regions, pathway assemblies, and long-fragment chromosomal segments.
Accuracy and sequence verification. Every synthesized gene is verified by Sanger sequencing prior to delivery, with 100% sequence accuracy guaranteed. Error-correction and clone screening are applied throughout the synthesis workflow to eliminate insertions, deletions, and substitutions — particularly critical for long fragments and repetitive sequences where synthesis errors accumulate.
Codon optimization. Free codon optimization is included with every gene synthesis order. The optimization algorithm accounts for host-specific codon preference, GC content, mRNA secondary structure, restriction enzyme site avoidance, and internal regulatory motifs. Supported expression hosts include E. coli, yeast (Pichia pastoris and S. cerevisiae), insect cells (Sf9/Hi5), CHO, HEK293, and other mammalian systems. Codon optimization consistently increases recombinant protein expression yields and solubility compared to native sequences.
Difficult and complex sequences. The synthesis platform is engineered to handle high-GC content sequences (>80% GC), repetitive elements, tandem repeats, homopolymer runs, and sequences with strong secondary structure — categories that routinely fail conventional synthesis pipelines. Complexity assessment is performed on each submitted sequence prior to synthesis to select the appropriate assembly strategy and accurately estimate turnaround time.
Deliverables. Synthesized genes are delivered cloned into a customer-specified or Enzovera-recommended vector, as sequence-verified plasmid in E. coli stab culture or as purified plasmid DNA. Lyophilized linear dsDNA delivery is available on request. Free vector storage is included.
Oligo Synthesis
Custom oligonucleotide synthesis covers the full spectrum of research and industrial oligo formats. The synthesis platform operates on solid-phase phosphoramidite chemistry with quality control by mass spectrometry and large-scale sequencing, verifying an average mutation rate below 1 per 1,000 bases.
DNA oligos. Standard unmodified DNA oligos for PCR, cloning, sequencing, and probe applications. Available from micro-scale to gram-quantity bulk. Length range: standard up to 150 nt; extended synthesis up to 300 nt on request. Purification options — desalting, RNase-free HPLC, PAGE — selected based on application and downstream sensitivity requirements.
RNA oligos and functional RNA. Custom RNA oligos, siRNA duplexes, miRNA mimics and inhibitors, sgRNA for CRISPR/Cas9, and antisense oligonucleotides (ASOs). siRNA and miRNA products are supplied as annealed, ready-to-transfect duplexes. sgRNA is available with 2′-OMe and phosphorothioate end modifications for increased intracellular stability.
Modified oligos. A comprehensive modification menu is available: 5′ and 3′ fluorescent labels (FAM, HEX, ROX, Cy3, Cy5, Texas Red, and others); quenchers (BHQ-1, BHQ-2, TAMRA); backbone modifications (phosphorothioate, LNA, 2′-OMe, 2′-F); internal modifications (dU/dI substitution, C3 spacers, biotin, amino linkers, disulfide linkers); and 5′ phosphorylation. Modifications can be combined and positioned at 5′, 3′, or internal sites. All modified oligos are subject to mass spectrometry QC prior to release.
Diagnostic probes and NGS oligos. High-specificity hybridization probes for FISH, ISH, and nucleic acid detection assays. NGS oligos — including adapters, capture probes, and index primers — synthesized to the stringent accuracy and purity specifications required by next-generation sequencing workflows.
CpG ODNs. Phosphorothioate-backbone CpG oligodeoxynucleotides for TLR9 agonism, immune activation research, and vaccine adjuvant development. Synthesized under optimized coupling conditions with full backbone modification verification.