Gene Synthesis & Molecular Services
Sequence to deliverable in 4–6 days.
Sanger-verified gene constructs, high-yield custom oligos, and tailored protein expression — all under one ordering relationship. Contractual turnaround times. No volume minimums.
Three services. One partner.
Submit a sequence, receive a verified construct, a quantified oligo pool, or a purified protein — with a certificate of analysis for every order.
Gene Synthesis
Double-stranded DNA constructs from 800 bp to 3,000 bp, cloned into your vector, fully Sanger-verified before shipment.
- Full Sanger verification + raw trace files with every order
- Codon optimization available on request
- Customer-supplied or catalog cloning vectors accepted
Custom DNA Oligos
High-purity custom oligonucleotides at OEM pricing, with desalt, PAGE, and HPLC purification options.
- Volume pricing for bulk and repeat orders
- Modified bases available on request
- COA with purity data per lot
Protein Expression
End-to-end protein production from gene design through expression, purification, and SDS-PAGE purity analysis.
- Yield and purity targets defined per project quotation
- Full S2P workflow available with gene synthesis
- COA including purity gel image with every order
Five steps from sequence to delivery.
No work begins without a signed Purchase Order. Every step is tracked and documented.
Submit Sequence
Email your sequence with vector preference and any codon optimization target.
Written Quote
Receive a written quotation within one business day. Signed PO required before work begins.
Synthesis & QC
Synthesized, cloned, colony-screened, and Sanger-verified.
COA & Data Package
Certificate of analysis, Sanger trace files, and sequence alignment shipped with every order.
Cold Delivery
Shipped cold to your designated lab address within 4–6 business days of order confirmation.
Built for your research workflow.
From CRISPR screening libraries to antibody engineering and vaccine development — our services integrate cleanly into demanding downstream workflows.
Antibody Engineering
Synthesize VH/VL variable region genes, humanization constructs, and bispecific scaffolds ready for CHO or HEK293 expression.
CRISPR & Gene Editing
High-purity sgRNA oligos, donor templates, and Cas9 expression vectors for precise genome editing workflows.
Vaccine & Antigen Research
Rapid synthesis of antigen-coding sequences for mRNA vaccine candidates, viral vector inserts, and subunit protein production.
Pathway Engineering
Construct metabolic pathways gene by gene with codon-optimized sequences tuned for your expression host.
Mutagenesis Libraries
Degenerate oligo pools and site-saturation libraries for deep mutational scanning and directed evolution campaigns.
High-Volume & Industrial
Bulk oligo production and repeat gene orders at volume pricing. Contact us for standing order arrangements.
Standard verification protocol.
Reference reaction setup and thermal profile for downstream Sanger verification of delivered gene constructs using Enzovera VeraDye™ reagents.
Reaction Setup — Sanger Verification (10 µL)
| Component | Volume |
|---|---|
| VeraDye™ Terminator Mix (1:3 diluted) | 2 µL |
| 5× Sequencing Buffer | 1 µL |
| Primer (3.2 µM) | 1 µL |
| Plasmid template (25–50 ng) | 2 µL |
| Deionized water | 4 µL |
| Total | 10 µL |
Use EV-SGR-002 VeraDye™ Kit for downstream verification. Compatible with all ABI Genetic Analyzer platforms.
DNA Template Input Guide
| Template Type | Input |
|---|---|
| PCR product 100–200 bp | 1–3 ng |
| PCR product 200–500 bp | 3–10 ng |
| PCR product 500–1,000 bp | 5–20 ng |
| Plasmid / dsDNA construct | 25–50 ng |
| Cosmid / BAC | 150–300 ng |
PCR Thermal Profile — Construct Verification (35 cycles)
For PCR product templates, reduce to 30 cycles. For hairpins or PolyG repeats, add EV-SGR-010 HP Hairpin Reagent.
Service specs at a glance.
Turnaround times are contractual service targets, not estimates. Repeated or material failure to meet them constitutes a material breach.
| Service | Turnaround | QC Method | Yield | Verified | COA |
|---|---|---|---|---|---|
| Gene Synthesis ≤2.3 kbEV-SYN-001 | 4–6 business days | Full Sanger | 2–5 µg | ✓ | ✓ |
| Gene Synthesis ≤3 kbEV-SYN-001 | 4–6 business days | Full Sanger | 2–5 µg | ✓ | ✓ |
| DNA Oligos — StandardEV-SYN-002 | 3–5 business days | MS / OD | Per spec | — | ✓ |
| DNA Oligos — PAGE/HPLCEV-SYN-002 | 5–7 business days | PAGE / HPLC | Per spec | — | ✓ |
| Protein ExpressionEV-SYN-003 | Project-dependent | SDS-PAGE + assay | Per quotation | — | ✓ |
Complete service listing.
All services are quote-based. Submit your sequence or project description to receive a written quotation within one business day.
| SKU | Service | Category | Scale / Format |
|---|---|---|---|
| EV-SYN-001 | Gene Synthesis | Custom Gene Construction | 800 bp – 3 kb |
| EV-SYN-002 | Custom DNA Oligos | Oligonucleotide Synthesis | 25 nmol – bulk |
| EV-SYN-003 | Protein Expression & Purification | Recombinant Protein Production | Quote-based |
Ready to place an order?
Send your sequence or describe your project. Written quotation within one business day.