Sanger Sequencing
Reagents
A complete portfolio of high-performance reagents, polymers, and purification solutions for first-generation DNA sequencing — engineered for throughput, reliability, and cost efficiency.
Precision at Every Step of Sanger Sequencing
The Enzovera Sanger Sequencing portfolio covers the entire first-generation sequencing workflow — from template preparation and cycle sequencing through cleanup, capillary electrophoresis, and data quality. Every product is independently QC-tested across 30-replicate runs using both standard and complex-structure plasmids, with batch data shared transparently at time of shipment.
Sequencing Reagents & Kits
Core terminator chemistry, buffers, and sequencing additives for standard, hairpin, and short-fragment sequencing workflows.
Capillary Electrophoresis Reagents
Separation polymers, running buffers, and sample preparation reagents engineered for ABI Genetic Analyzer platforms.
Template Prep & Cleanup Reagents
Three-method cleanup portfolio with the industry's fastest resin-based cleanup at under 10 minutes, plus magnetic bead solutions for automated workflows.
From Template to Sequence — One Portfolio
Standard Sequencing Protocols
Reaction Setup (10 µL System)
| Component | Volume |
|---|---|
| Working enzyme mix (1:3 diluted) | 2 µL |
| Primer (3.2 µM) | 1 µL |
| Deionized water | 5 µL |
| Template DNA | 2 µL |
| Total | 10 µL |
Template DNA Quantity Guide
| Template Type | Recommended Amount |
|---|---|
| PCR product 100–200 bp | 1–3 ng |
| PCR product 200–500 bp | 3–10 ng |
| PCR product 500–1,000 bp | 5–20 ng |
| Plasmid / double-stranded DNA | 25–50 ng |
| Cosmid / BAC | 150–300 ng |
| Bacterial genomic DNA | 0.5–1.0 µg |
PCR Thermal Profile (Plasmid, 35 cycles)
For PCR product templates reduce to 30 cycles. For complex structures (hairpins, PolyG), use EV-SGR-010 HP reagent. Ramp rate: 1°C/sec.
Batch Quality Control & Transparency
30-Replicate QC Standard
Each batch of sequencing enzyme is tested in 30 replicate runs on both BS-Plasmid-A (standard structure) and BS-Plasmid-B (complex structure with multi-segment PolyG repeats). Release requires all key parameters within 90–110% of the reference standard.
Shared QC Data at Shipment
Internal QC data (192 samples, 2 plates) plus external validation data from at least one independent Sanger sequencing facility accompanies every shipment. Customers may query any batch's historical data at any time.
Key Performance Metrics
Parameters tracked include peak heights at 100 bp, 500 bp, and 800 bp; average signal across all four bases; effective read length; and background peak ratio. Complex templates additionally tracked at three PolyG segment positions.
Complete Product Listing
| SKU | Product Name | Category | Pack Size |
|---|---|---|---|
| EV-SGR-002 | VeraDye™ Terminator v3.1 Cycle Sequencing Kit | Sequencing Kit | 100 rxn · 500 rxn · 1,000 rxn |
| EV-SGR-003 | 5× Sequencing Buffer | Sequencing Reagent | 5 ml · 30 ml |
| EV-SGR-010 | HP Hairpin Reagent (7-deaza dATP) | Sequencing Additive | 1 ml/tube |
| EV-SGR-012 | Phi29 Rolling Circle Amplification Kit | Template Amplification | 50 rxn · 200 rxn |
| EV-SGR-004 | POP-7 Separation Polymer | CE Polymer | 28 ml/bottle |
| EV-SGR-005 | POP-4 Separation Polymer | CE Polymer | 3.5 ml/bottle |
| EV-SGR-006 | 10× Running Buffer with EDTA | CE Running Buffer | 500 ml/bottle |
| EV-SGR-011 | Hi-Di Formamide | CE Sample Prep | 25 ml · 500 ml |
| EV-SGR-008 | Sequencing Cleanup Resin | Sanger Cleanup | 50 ml · 100 ml · 1 L |
| EV-SGR-007 | Sequencing Product Purification Magnetic Beads | Sanger Cleanup | 10,000T/tube |
| EV-SGR-009 | Template Preparation Magnetic Beads v3 | Template Prep | 10,000T/tube |